In-house calibration and control of piston operated pipettes (NT TR 626)

Abstract

Volumetric apparatus are commonly used in laboratories for addition of specific amounts of liquids such as reagents, solvents and samples. This Nordtest technical report presents three procedures for piston operated volumetric apparatus used in routine laboratories:
  1. In-house calibration;
  2. Verification of specification; and
  3. Routine check.
Detailed guidance is presented for pipettes for volumes from 10 μL to 10 mL but the procedures presented here are also applicable to burettes, dilutors, dispensers and manually operated laboratory syringes. Important references are;
  • ISO 8655-1 [3] giving general requirements and terms for volumetric measuring apparatus;
  • ISO 8655-2 [4] and ISO 8655-6 [5] describes gravimetric methods for determining the volume delivered from piston operated volumetric apparatus; and
  • ISO 8655-10 [6] giving user guidance.